第7回 Student Monthly Progressを開催しました
10月27日 (金)、第7回 Student Monthly Progressを開催しました。
The 7th Student Monthly Progress presentation was held on Friday, October 27th, 2023.
【発表者 / Presenters】
1. THAMMAHAKIN Passawat (D2): Laboratory of Public Health
Detection of disease-associated-microglia among various microglia phenotypes induced by West Nile virus infection in mice
2. RUBEL Md. Zahir Uddin (D2): Laboratory of Anatomy
Lymphoid tissues in chicken rectal mucosa and factors affecting its morphology
3. PANDIT Arjun (D2): Laboratory of Wildlife Biology and Medicine
Elephant Health Crisis: Tuberculosis related mortality in Nepal, 2019-2022
4. SATO Junpei (D2): Laboratory of Infectious Diseases
Insertion in the Meq protein of the avian oncogenic herpesvirus, Marek’s disease virus, accelerates the process of tumorigenesis.
【座長 / Chairpersons】
NESTI Dela Ria (D2): Division of Collaboration and Education
WATANABE Kanami (D2): Laboratory of Toxicology
❖座長レポート Chairpersons’ Report ❖
Passawat Thammahakin’s research is focused on identifying a range of microglia phenotypes in West Nile virus (WNV)-infected mice
and disease-associated microglia that may influence WNE pathogenicity. He inoculated mouse WNV (NY-99 strain) and collected the
brain to investigate the variety of microglia phenotypes by using marker molecules with immunohistology analysis. He also used
immunofluorescent and flow cytometry to determine the phenotype interacting with WNV-infected neurons. As a result, He detected
the positive cells of the general microglia marker in both the control and WNV-infected brains, but the morphology was different.
He concluded that WNV infection can induce activation of diverse microglia phenotypes and the disease-associated microglia may be
associated with the pathogenicity of WNV infection in the mouse brain.
In his research, Md. Zahir Uddin Rubel’s explored rectal mucosa-associated lymphoid tissue (RMALT) morphology and related variables
in various ages and sexes of chickens. He used histological observation and ultrastructure scanning to analyze the rectums of Rhode
Island Red (RIR) chickens of different ages. As an outcome, he discovered that the total lymphoid tissue count in adults was
substantially larger than in chicks. Adult chickens have much bigger RMALT sizes in the rectum than 1-day and 1-month chicks.
Furthermore, the mean size of total lymphoid tissues was considerably greater in adult females than in men for sex-related differences.
He summarized that age and sex both influenced the placement of lymphoid tissues and immune cell composition in the rectum.
Arjun Pandit’s research focuses on Tuberculosis-related mortality in Nepal from 2019 to 2022. Tuberculosis poses a significant threat
to elephants in Nepal, primarily caused by Mycobacterium tuberculosis. This study aimed to investigate the genetic characteristics of
the pathogen responsible for elephant deaths. Testing confirmed the presence of tuberculosis in some elephants, and the analysis
indicated possible disease transmission between elephants and humans. The rising prevalence of a specific strain raised concerns
regarding drug resistance.
Jumpei Sato’s presentation explores the impact of an insertion in the Meq oncoprotein of Marek's disease virus (MDV) in chickens.
Despite vaccination, MDV strains have become more virulent. The insertion (L-Meq) was found to enhance Meq's activity in transactivation
assays. Recombinant MDVs with L-Meq had higher mortality and tumor incidence, as well as increased CD4+ T cells after infection.
However, there were no significant differences in gene expression in tumor lesions. In summary, the Meq insertion accelerates
tumorigenesis and increases virulence by enhancing transactivation activity without affecting target gene regulation.
This event provided a glimpse into the diverse research pursuits of fellow PhD students across various fields. The students actively
engaged by asking thought-provoking questions, sparking dynamic discussions with the presenters. Involvement in such activities
allows students to expand their research horizons beyond their respective domains and cultivates a collaborative research mindset.